| SLC30A3

Zinc transporter 3 also known as solute carrier family 30 member 3 is a protein that is encoded by the SLC30A3 gene.The SLC30A3 gene codes for the ZnT-3 SLC30A family membrane transport protein. ZnT-3 is required for the accumulation of zinc ions inside synaptic vesicles. Zinc transport by ZnT-3 modulates memory formation by acting through the extracellular signal-regulated kinases signaling pathway.Angiotensin II-induced senescence of vascular smooth muscle cells requires down-regulation of ZnT-3 and ZnT-10.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.36	1.79	1.28	0.86	0.63	0.38	0.26	0.08
Corrected OD	2.28	1.71	1.2	0.78	0.55	0.3	0.18	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse SLC30A3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	94-105	102
Cell Culture Supernates(n=8)	96-108	103

 

| Linearity

Samples were spiked with high concentrations of Mouse SLC30A3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	88-96	92-110
	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
	Average (%)	98	110